FACILITY & INSTRUMENTATION

East side of Bonner Hall Lower Level.

The Cryo-EM Facility at UCSD is located on the lower level of Bonner Hall on campus in La Jolla, California.


Electron Microscopes

Titan Krios G3

300 keV, FEG, Autoloader, with Gatan K2 direct electron detector, Gatan Energy Filter and volta phase plates.

This cryo-STEM microscope is equipped with a 3 condenser lens system allowing for a wide range of beam settings while maintaining parallel illumination.

Currently single particle and tomography data collection is automated through Leginon, EPU or SerialEM packages.

TeCNAI G2 POlara

300 keV, FEG, Multi-specimen holder, with DE-20 direct electron detector. Automation through SerialEM.

Talos Arctica

200 keV, FEG, Autoloader, with Gatan K2 direct electron detector and phase plate. Automation through Leginon.

TECNAI G2 SPHERA

200 keV, LaB6 filament, with Gatan 4k x 4k CCD. Automation through Leginon.

Aquilos Cryo-FIB

A focused ion beam/scanning electron microscope (FIB-SEM) dedicated to the preparation of frozen, thin lamella samples from biological specimens for high-resolution tomographic imaging in a cryo-transmission electron microscope (cryo-TEM).

Specimen Preparation

Plunge Freezing

  • FEI Vitrobot (Mark IV x2)

  • Manual Apparatus (Cold room)

Glow Discharge

  • Gatan Solarus II (Installation Spring 2019)

  • Emitech K100X

Carbon Coating

  • Edwards Auto 306

  • Safematic (Installation Summer 2019)

Wet Lab

  • Negative Stain and Specimen Prep materials.

People

Tim Booth

Cryo-EM Project Specialist

  • Instrumentation training and maintenance.

  • Facility Operations