FACILITY & INSTRUMENTATION
The Cryo-EM Facility at UCSD is located on the lower level of Bonner Hall on campus in La Jolla, California.
Titan Krios G3
300 keV, FEG, Autoloader, with Gatan K2 direct electron detector, Gatan Energy Filter and volta phase plates.
This cryo-STEM microscope is equipped with a 3 condenser lens system allowing for a wide range of beam settings while maintaining parallel illumination.
Currently single particle and tomography data collection is automated through Leginon, EPU or SerialEM packages.
TeCNAI G2 POlara
300 keV, FEG, Multi-specimen holder, with DE-20 direct electron detector. Automation through SerialEM.
200 keV, FEG, Autoloader, with Gatan K2 direct electron detector and phase plate. Automation through Leginon.
TECNAI G2 SPHERA
200 keV, LaB6 filament, with Gatan 4k x 4k CCD. Automation through Leginon.
A focused ion beam/scanning electron microscope (FIB-SEM) dedicated to the preparation of frozen, thin lamella samples from biological specimens for high-resolution tomographic imaging in a cryo-transmission electron microscope (cryo-TEM).
FEI Vitrobot (Mark IV x2)
Manual Apparatus (Cold room)
Gatan Solarus II (Installation Spring 2019)
Edwards Auto 306
Safematic (Installation Summer 2019)
Negative Stain and Specimen Prep materials.
Cryo-EM Project Specialist
Instrumentation training and maintenance.